Stimulation of glucose catabolism in Escherichia coli by a potential futile cycle. Patnaik R, WD Roof, RF Young and JC Liao. Engineering of Escherichia coli central metabolism for aromatic metabolite production with near theoretical yield. In: A Short Course in Bacterial Genetics, pp 268–274, Cold Spring Harbor Laboratory, Cold Spring Harbor. Production of phenylalanine and organic acids by phospho enolpyruvate carboxylase-deficient mutants of Escherichia coli. Miller JE, KC Backman, MJ O'Conner and RT Hatch. Deletion of pgi alters tryptophan biosynthesis in a genetically engineered strain of Escherichia coli. Low copy number plasmids for regulated low-level expression of cloned genes in Escherichia coli with blue/white insert screening capability. Cloning of the pyruvate kinase gene ( pyk) of Corynebacterium glutamicum and sitespecific inactivation of pyk in a lysine-producing Corynebacterium lactofermentum strain. Gubler M, M Jetten, SH Lee and AJ Sinskey. Prospects for biocatalytic synthesis of aromatics in the 21 st century. Nature Biotechnol 14: 620–623.įrost JW and JC Lievense. Pathway engineering for the production of aromatic compounds in Escherichia coli. Science 222: 167–169.įlores N, J Xiao, A Berry, F Bolivar and F Valle. Expression of naphthalene oxidation genes in Escherichia coli results in biosynthesis of indigo. J Am Chem Soc 114: 9725–9726.Įnsley BD, BJ Ratzkin, TD Osslund, MJ Simon, LP Wackett and DT Gibson. Biocatalysis and nineteenth century organic chemistry: conversion of d-glucose into quinoid organics. J Am Chem Soc 114: 3956–3962.ĭraths KM, TL Ward and JW Frost. Biocatalytic synthesis of aromatics from d-glucose: the role of transketolase. Bio/Technology 8: 634–638.ĭraths KM, DL Pompliano, DL Conley, JW Frost, A Berry, GL Disbrow, RJ Staversky and JC Lievense. Melanin production in Escherichia coli from a cloned tyrosinase gene. J Bacteriol 134: 1141–1156.ĭella-Cioppa G, SJ Garger, GG Sverlow, TH Turpen and LK Grill. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. Construction and characterization of new cloning vehicles. Golden, Colorado.īolivar F, RL Rodriguez, PJ Greene, MC Betlach, HL Heyneker and HW Boyer. In: Proceedings of the Second Biomass Conference of the Americas: Energy, Environment, Agriculture, and Industry, pp 1121–1129, National Renewable Energy Laboratory. coli: development of a biological system for the cost-effective production of a large volume chemical.
Biosynthesis of indigo using recombinant E. Cellular and Molecular Biology, Vol 2 (Neidhardt FC, JL Ingraham, KB Low, B Magasanik, M Schaechter and HE Umbarger, eds), pp 1444–1452, American Society for Microbiology, Washington, DC.īerry A, S Battist, G Chotani, T Dodge, S Peck, S Power and W Weyler. In: Escherichia coli and Salmonella typhimurium. When all of the variables tested (PTS − glucose +, pykA, pykF, and overexpressed tktA) were combined in a single strain, a 19.9-fold increase in carbon commitment to aromatic biosynthesis was achieved.īeckwith J. In the PTS − glucose + host background, overexpression of tktA caused a further 3.7-fold increase in carbon flow, while inactivation of pykA and pykF caused a 5.8-fold increase. A PTS glucose + mutant showed a 1.6-fold increase in carbon flow to aromatics compared to the PTS + control strain. In a strain already having increased carbon flow to aromatics by virtue of overexpression of the tktA gene (encoding transketolase), the pykA and/or pykF mutations had no effect. In a strain having a wild-type PEP: glucose phosphotransferase (PTS) system, inactivation of the genes encoding pyruvate kinase ( pykA and pykF) resulted in a 3.4-fold increase in carbon flow to aromatic biosynthesis.
Different approaches to increasing carbon commitment to aromatic amino acid biosynthesis were compared in isogenic strains of Escherichia coli.
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